Effects of Carbosulfan Administration Schedules on Estrous Cycle and Follicular Dynamics in Albino Mice

Abstract

Carbosulfan an acaricide was orally administrated at 48 mg/kg body weight/day to female swiss albino mice for 5, 10, 20 and 30 d. Daily vaginal smear and body weight was recorded. The mice were sacrificed by cervical dislocation after 24 h of terminal exposure to carbosulfan. The results of the present study indicated that there was a significant decrease in the number of estrous cycle and phases with a concomitant increase in the length of the estrous cycle and diestrus phase with carbosulfan treatment for 10, 20, and 30 d. There was a significant decrease in the weight of the ovary, uterus, number of healthy follicles and increase in the number of atretic follicles with 20 and 30 d carbosulfan treatment when compared with the control. There was a significant decrease in the level of sialic acid with an increase in the level of cholesterol by carbosulfan treatment for 20 and 30 d. The decreased level of sialic acid indicates the decreased level of circulating FSH/LH. The ovary 3beta HSD activity decreased significantly with 20 and 30 d carbosulfan exposure mice indicating its effect on steroidogenesis, when compared to controls. The above findings may be due to direct effect on the ovary or on the gonadotrophins secretion via central nervous mechanism in mice.