Effects of captlpril on proliferation of rat hepatic stellate cells

Abstract

Objective To investigate the effects of angiotensin Ⅱ (Ang Ⅱ) and captlpril on proliferation of culured hepatic stellate cells in vivo.Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated HSCs.Methyl thiazol tetrazolium (MTT) colorimetric assay was used to e valuate the proliferation of HSCs.Results A570 value of control groups was 52.28 ± 1.19 ; A570 values of Ang Ⅱ groups (1 × 10-5 mol/L,1 × 10-7 mol/L,1 × 10-9 mol/L) were 74.81 ±3.61,61.76 ± 1.34,58.12 ± 1.29.A570 values of Captlpril + Ang Ⅱ groups (1 × 10-5 mol/L,1 × 10-7 mol/L,1 × 10-9 mol/L) were 36.32 ±2.11,41.36 ± 1.09,47.01 ±2.65.A570 value of Captlpril + Ang Ⅱ groups was lower than that of control groups (P ＜ 0.01).A570 value of Ang Ⅱ groups was higher than that of control groups (P ＜ 0.01).Conclusion Angiotensin Ⅱ (1 × 10-9 to 1 × 10-5 mmol/L) stimulated proliferation of HSCs as demonstrated by MTT assay.Captlpril + Ang Ⅱ had significantly inhibitory effect on growth of HSCs at the concentration of 1 × 10-9 to 1 × 10-5 mmol/L. Key words: Angiotensin Ⅱ ; Captlpril; Hepatic stellate cells; Proliferation