Effects of Calyx Fluid from a Population of Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae) on the Immune Response of its Host Busseola fusca Fuller (Lepidoptera: Noctuidae)

Abstract

Busseola fusca Fuller is one of the major lepidopteran stemborers of maize and sorghum in Africa. Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae) is an indigenous, gregarious larval endoparasitoid that attacks mid- to late-instar stemborer larvae, which is reported to be one of the most important larval parasitoids of B. fusca in many countries in sub-Saharan Africa. Previous work has shown that two biotypes of C. sesamiae occur in Kenya which express differential abilities to develop in B. fusca. A biotype from western Kenya successfully develops in B. fusca, while a biotype from the eastern coastal area does not. We treated fourth-instars of B. fusca with calyx fluid from C. sesamiae from western Kenya (Kitale) before offering them to C. sesamiae from the Coast (Mombasa) for oviposition, and found that the eggs were not encapsulated. This suggests that factors in the calyx fluid of C. sesamiae from the western Kenya biotype were responsible for suppressing the immune system of B. fusca. We speculate that polydnaviruses in the calyx fluid were involved in disarming the host immune system, and that the polydnaviruses in the two biotypes are genetically different.