Effects of calreticulin mediated apoptosis during the early threatened abortion

Abstract

Objective To explore the relationship between the expression level of calreticulin (CRT) and early threatened abortion by observing CRT expression level in decidua and villi tissues. Methods 1) The decidua and villi tissues of normal pregnancies and early threatened abortion patients were collected and treated respectively. 2) The expression of CRT protein was verified by immunohistochemistry and Western blotting, and the expression of CRT mRNA was detected by qPCR. 3) The plasmid mEmerald-CRT was synthesized and detected. 4) CRT-siRNA was synthesized and detected. 5) Plasmid mEmerald-CRT and CRT-siRNA were transfected into BeWo cells respectively and the expressions of CRT and Caspase-3 were detected by Western blotting and qPCR. Results 1) The decidua and villi tissues of normal pregnancies (35 cases) and early threatened abortion (40 cases) were collected. 2) Immunohistochemistry showed that the stains were deeper and bigger in early threatened abortion group than the normal pregnancy group; qPCR test and Western blotting showed the expression of CRT was significantly higher in early threatened abortion than that in normal pregnancy, the expression of mRNA in early threatened abortion groups was 1.2 times of normal pregnancy (P<0.05). 3) qPCR test and Western blotting showed CRT and Caspase-3 in mEmerald-CRT groups were significantly higher than those in control groups, the expressions of CRT and Caspase-3 in mEmerald-CRT groups were 3.5 times and 2 times of control groups respectively (P<0.05). 4) qPCR test and Western blotting showed CRT and Caspase-3 in CRT-siRNA groups were significantly lower than those in control groups, the expressions of CRT and Caspase-3 in CRT-siRNA groups were 5 times and 2 times of control groups, respectively (P<0.05). Conclusion The occurrence of early threatened abortion may be related to excessive CRT expression and its mediated abnormal apoptosis in decidua and villi tissues. Key words: Calreticulin; Decidua; Villi; Early threatened abortion; Cell apoptosis