Abstract
The cellular and metabolic effects induced by calcitonin on regenerative epidermal cells, fibroblasts and collagen fibers during wound healing were studied in rabbits. Electron microscopy revealed marked changes at 2 and 5 days post-wounding in epidermal cells and fibroblasts in calcitonin-treated rabbits such as: an increase in keratohyaline granules, tonofilaments, polysomes and a hypertrophy of nuclei in the epidermal cells. Fibroblasts are hypertrophied and surrounded by large areas of mature collagen fibers, namely at 5 and 14 days. Electron microscopic autoradiography revealed an increase in the incorporation of 3H-thymidine mostly in the nuclear chromatin, 3H-uridine in the nucleoli, 3H-leucine in the endoplasmic reticulum, polysomes and newly formed keratin layers, and 3H-proline over fibroblasts and collagen fibers. Most of the cellular and metabolic effects are evident at 2.5 hours and gradually decline by 5 and 24 hours of hormone administration. Scanning electron microscopy showed changes in the keratin pattern and collagen fibers. At 5 days after wounding and calcitonin treatment, a typical scaly pattern of keratin can be noted while in controls it is smooth and homogeneous. At 14 days post-wounding the collagen fibers are hypertrophied, with an extensive network of fibrils in calcitonin-treated rabbits. These findings demonstrate that synthetic salmon calcitonin stimulates protein synthesis, keratinogenesis and collagen formation in epidermal cells and fibroblasts during wound healing.
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