Effects of caffeoylxanthiazonoside on airway inflammation in an allergic asthma mice model

Qian Wu,Wei Wang,Hui Wang,Xiaowen Che

doi:10.4314/tjpr.v18i4.12

Abstract

Purpose: To investigate the inhibitory effects of caffeoylxanthiazonoside (CYT) on airway inflammation in mice and its mechanism of action. Methods: An allergic asthma mice model was established by intraperitoneal injection and aerosol nebulization with ovalbumin (OVA). After treatment with CYT, the blood and bronchoalveolar lavage fluid (BALF) were collected from the mice. The leukocytes were classified and counted with Giemsa solution. Enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of IgE, and IL-4, IL-5, IL-13 and IFN-γ in the BALF of mice. Lung tissues were obtained from the mice and MUC5AC protein expression was measured by western blot. Results: CYT significantly decreased the serum level of IgE in asthmatic mice. Inflammatory cells in BALF of mice were markedly reduced (p < 0.05) by CYT treatment at varying doses (10, 20, and 40 mg/kg). Treatment with CYT also significantly suppressed the cytokines of IL-4, IL-5 and IL-13 and increased the IFN-γ in the BLAF of OVA-induced allergic asthma mice (p < 0.05). Western blot results indicate that CYT treatment significantly decreased the expression of MUC5AC protein in the lung tissues of asthmatic mice. In addition, no significant effects on the body weight of the mice were found after CYT treatment. Conclusion: Caffeoylxanthiazonoside inhibits airway inflammation in allergic asthma mice by altering Th1/Th2 via re-balancing of related cytokines and downregulation of lung MUC5AC protein expression. Therefore, this compound can potentially be developed for the therapeutic management of inflammation in allergic asthma.

Highlights

Asthma is a chronic airway inflammatory disease with the characterizations of reversible airflow obstruction, goblet cell and smooth muscle hyperplasia, increased mucus production, hyperactivity and ultrastructural remodeling of the airways [1,2]
Caffeoylxanthiazonoside (CYT, Figure 1) is an active thiazinedione heterocyclic compound isolated from the fruits of Xanthium strumarium L. (XSL), and investigations have shown that CYT possesses favorable therapeutic effects on allergic rhinitis, sepsis, and chronic heart failure [9,10]
The serum levels of IgE, and inflammatory cytokines including IL-4, IL-5, IL-13 and IFN-γ in the bronchoalveolar lavage fluid (BALF) of mice were measured by commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions

Summary

INTRODUCTION

Asthma is a chronic airway inflammatory disease with the characterizations of reversible airflow obstruction, goblet cell and smooth muscle hyperplasia, increased mucus production, hyperactivity and ultrastructural remodeling of the airways [1,2]. Caffeoylxanthiazonoside (CYT, Figure 1) is an active thiazinedione heterocyclic compound isolated from the fruits of XSL, and investigations have shown that CYT possesses favorable therapeutic effects on allergic rhinitis, sepsis, and chronic heart failure [9,10]. The aim of this study was ti determine the effects of CYT on airway inflammation in an allergic asthma mice model. The left lungs of the mice were lavaged with 0.5 mL normal saline to obtain the BALF. The serum levels of IgE, and inflammatory cytokines including IL-4, IL-5, IL-13 and IFN-γ in the BALF of mice were measured by commercial ELISA kits according to the manufacturer’s instructions. CYT at different doses had significant inhibitory effect on inflammatory cells in mice BALF (Figure 3).

RESULTS

CONCLUSION

DISCUSSION

Conflict of interest