EFFECTS OF CAFFEIC ACID PHENETHYL ESTER ON THE EXPRESSION OF INTERLEUKINS 6 AND 8 IN ORAL SQUAMOUS CELL CARCINOMA

Abstract

Objective The present study evaluated the effects of caffeic acid phenethyl ester (CAPE) on cell viability and expression of interleukins (IL-) 6 and 8 in oral squamous cell carcinoma cell cultures (CAL-27). Study Design CAL-27 cells were plated at the density of 3500 cells/well in 96-well plates and exposed to CAPE at concentrations of 0, 25, 50, 100, and 200 μM. After 24, 48, and 72 hours, the following parameters were evaluated: (1) cell viability by MTT and (2) expression of IL-6 and IL-8 by enzyme-linked immunosorbent assay (ELISA). Data were submitted to the Kruskal-Wallis test, followed by Student-Newman-Keuls test (α = 5%). Results CAL-27 cultures exposed to CAPE exhibited a reduction in cell viability compared to the nonexposed CAL-27 cultures (P < .05). The lowest cell viability was identified in cultures exposed to 200 μM CAPE in all experimental time points evaluated (P < .05). The highest IL-6 and IL-8 levels were detected in CAL-27 cultures exposed for 48 hours to 100 μM CAPE (P < .05). Conclusions The results indicate that CAPE is able to reduce cell viability and modulate the expression of IL-6 and IL-8 in CAL-27 cultures. The present study evaluated the effects of caffeic acid phenethyl ester (CAPE) on cell viability and expression of interleukins (IL-) 6 and 8 in oral squamous cell carcinoma cell cultures (CAL-27). CAL-27 cells were plated at the density of 3500 cells/well in 96-well plates and exposed to CAPE at concentrations of 0, 25, 50, 100, and 200 μM. After 24, 48, and 72 hours, the following parameters were evaluated: (1) cell viability by MTT and (2) expression of IL-6 and IL-8 by enzyme-linked immunosorbent assay (ELISA). Data were submitted to the Kruskal-Wallis test, followed by Student-Newman-Keuls test (α = 5%). CAL-27 cultures exposed to CAPE exhibited a reduction in cell viability compared to the nonexposed CAL-27 cultures (P < .05). The lowest cell viability was identified in cultures exposed to 200 μM CAPE in all experimental time points evaluated (P < .05). The highest IL-6 and IL-8 levels were detected in CAL-27 cultures exposed for 48 hours to 100 μM CAPE (P < .05). The results indicate that CAPE is able to reduce cell viability and modulate the expression of IL-6 and IL-8 in CAL-27 cultures.