Effects of Ca2+-activated K + channels in epithelial-mesenchymal transition induced by phosphatase of regenerating liver-3 in Lovo cells

Abstract

Objective To investigate the effects of Ca2+-activated K + channels (KCNN4) in the epithelial-mesenchymal transition (EMT) induced by phosphatase of regenerating liver-3 (PRL-3) in Lovo cell line.Methods Lovo-P cells were treated with Tram-34 and KCNN4-small interfering RNA (siRNA)respectively.Western blotting was used to detect the E-cadherin,Snail and Vimentin protein levels.The invasion of Lovo cells was assessed by Transwell invasion assay.Results Mter the Lovo ceils were transfected with PRL-3,the invasion ability was significantly enhanced.At the same time,the expression of E-cadherin was inhibited (P ＜ 0.05) and that of Vimentin was increased (P ＜ 0.05).When cells were treated with Tram-34 and KCNN4-siRNA,the invasion ability of Lovo cells was inhibited significantly (45.78 ±3.89 vs.32.69 ± 2.15 ; 45.78 ± 3.89 vs.28.76 ± 4.25,P ＜ 0.05).Consequently,the expression of E-cadherin was increased by 69％ (P ＜ 0.05) and that of Vimentin was inhibited by 36％ (P ＜ 0.05) when the cells were treated with Tram-34.Similarly,the expression of E-cadherin was increased by 57％ (P ＜ 0.05) and that of Vimentin was inhibited by 30％ (P ＜ 0.05) when the cells were treated by KCNN4-siRNA.Conclusion KCNN4 may participate in the EMT induced by PRL-3 in Lovo cells. Key words: Colorectal cancer; Phosphatase of regenerating liver-3 ; Ca2+-activated K+ channels; Epithelial to mesenchymal transition