Abstract
Mouse mastocytoma cells were cultured with brefeldin A in medium containing [ 35S]sulfate and [ 3H]glucosamine in order to determine the effects of this fungal metabolite on the formation of chondroitin 4-sulfate by these cells. There was a marked reduction in the incorporation of [ 35S]sulfate into the glycosaminoglycan which was approximately equal to the reduction in the incorporation of [ 3H]hexosamine into the same molecule. The chondroitin 4-sulfate chain size was greatly diminished, while the number of chains appeared to remain relatively constant, indicating that the brefeldin A partially disrupted the polymerizing system, but had little effect upon movement of the nascent proteochondroitin to the site for chondroitin polymerization and sulfation.
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