Effects of brassinosteroid and brassinosteroid mimic on photosynthetic efficiency and rice yield under heat stress

Abstract

Brassinosteroids (BRs) have been reported to counteract various stresses. We investigated effects of exogenously applied brassinosteroid, 24-epibrassinolide (EBR), and brassinosteroid-mimic compound, 7,8-dihydro-8α-20-hydroxyecdysone (DHECD), on the photosynthetic efficiency and yield of rice (Oryza sativa L. cv. Pathum Thani 1) under heat stress. Solutions (1 nM) of EBR and DHECD were separately sprayed onto foliage of individual rice plants during their reproductive stage. Five days after the application, the plants were transferred to the day/night temperature regime of 40/30°C for 7 days and then allowed to recover at normal temperature for 7 days. We demonstrated that both DHECD and EBR helped maintain the net photosynthetic rate. The DHECD and EBR application enhanced stomatal conductance, stomatal limitation, and water-use efficiency under the high-temperature regime. DHECD- and EBR-treated plants showed an increase in the nonphotochemical quenching that was lower than that in the control plants. Moreover, DHECD and EBR treatments maintained the maximal quantum efficiency of PSII photochemistry and the efficiency of excitation capture of the open PSII center. Furthermore, the treatments with DHECD or EBR resulted in higher chlorophyll content during the heat treatment compared with the control plants. The paddy field application of 1 nM EBR and/or 1 nM DHECD at the reproductive stage during the hot season could increase the rice yield, especially, the number of filled seeds. DHECD and EBR enhanced total soluble sugar and reducing sugar in straw and more starch was accumulated in rice seeds. Consequently, our results confirmed that DHECD showed biological activities mimicking EBR in the improvement of photosynthetic efficiency and in rising the rice yield under heat stress.