Abstract
Male albino rats (90-100 g) were fed for 21 days a diet low in nitrogen (7.6%amino acids), devoid of valine, leucine and isoleucine and supple mented with branched-chain a-keto acids (9.4%; three times the minimal daily requirement of the corresponding amino acids). Pair-fed controls received an isocaloric diet which contained the three branched-chain amino acids (once the minimal daily requirement) instead of the a-keto acids. All other nutrients were the same in both diets. On day 3, 10 and 21 of the experiment, branched-chain a-keto acid dehydrogenase and branched-chain amino acid transaminase were measured in rat brain, kidney and liver. Glutamate dehydrogenase was deter mined in liver only. Ammonia was assayed in plasma, urea in plasma and urine. Plasma and urine urea decreased significantly when changing from the standard diet to both experimental diets, whereas the blood ammonia level remained fairly constant throughout the experiment. Feeding the experimental diets led to a 10-foldor greater decrease in hepatic branched-chain a-keto acid dehydrogenase activity. No such drastic response to the experimental diets was observed in other organs. Branched-chain amino acid transaminase and glutamate dehydrogenase were not influenced significantly by the dietary treatment. J. Nutr. 110: 1709-1716, 1980.
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