Effects of branched M2e polypeptides influenza A vaccine immunized through different routes

Abstract

Objective To compare the immune effects induced with universal influenza A vaccine based on branched M2e polypeptides by two immunization routes in mice. Methods The M2e monomer and (M2e)4-tuftsin were synthesized using chemical method. One group of mice were immunized intramuscularly (i.m.) with 10 μg of (M2e)4-tuftsin plus aluminum adjuvant. And the other group of mice were intranasally immunized with the same amount of polypeptides plus JY adjuvant. Then the immune effects of different immune pathways were observed. Results Intramuscular route induced higher level of M2 specific antibody than intranasal route. Intramuscular injection of (M2e)4-tuftsin was the most effective in stimulating T cell responses. Both intramuscular and intranasal vaccinations (M2e)4-tuftsin had protective effect, but intramuscular vaccination could provide the most efficient protective immunity. Conclusion The data indicated that the intramuscular vaccination could be the better way for immunization than intranasal vaccination. Key words: Peptides; Vaccines, DNA; Adjuvants, immanongic; Immunity, mucosal; Immunization, secondary; Influenza A virus