Effects of bound phenolic from defatted Moringa oleifera seed flour on diet-induced hypercholesterolemic mice

Abstract

The effect of bound phenolic and catechins extracted from defatted Moringa oleifera seed (DMSF) flour on diet-induced hypercholesterolemia was evaluated in this study. The animals (C57BL6 mice) were divided into four groups: group one received AIN 93M diet (control) group two received high-fat cholesterol diet (HFCD), three and four received HFCD along with bound phenolic and catechins, respectively, through oral administration at the dose of 25 mg/kg body weight daily for 8 weeks. Blood and tissue analysis showed that bound phenolic and catechins were able to reduce plasma and liver cholesterol significantly (p < .05). And these extracts were also able to reduce stress enzymes and molecules level in liver. The bound phenolic and catechins extracts from DMSF were able to increase plasma HDL level and lower atherogenic index in the treated groups. The data suggests that the combination of HFCD and bound phenolic or catechins extracted from DMSF as a source of antioxidants was beneficial. Practical applications In the present study the bound phenolic and catechins extracted from DMSF was studied for its effect on reducing cholesterol. DMSF is a processing waste left over after extraction of oil, hence prove to be cost-effective. In this study catechins (catechin and epicatechin) as well as combination of catechin-rich bound phenolic containing gallic acid, protocatechuic acid, ferulic acid, and catechin gallate were studied for its hypocholesterolemic effect. The results revealed that the combination of bound phenolic or catechins were comparably beneficial in reducing cholesterol and atherogenic index in diet induced mice model, which is the critical risk factor for atherosclerosis.