Abstract

ABSTRACT Raspberry (Rubus idaeus L.) explants were cultured in vitro under two boron concentrations (0.1 and 0.5 mM) and three levels of NaCl salinity (0, 5, and 10 mM) on an MS medium. The high boron and salt treatment (0.5 mM B and 10 mM NaCl) diminished mean shoot length and fresh and dry weights of the explants; however, fresh-to-dry weight ratio was positively affected. When B increased in the medium, leaf chlorophyll content and fluorescence were reduced under saline conditions. The absorption of boron, chloride, and sodium was enhanced by increased concentrations of boron and NaCl in the medium. Moreover, B enhanced the uptake and accumulation of chloride and sodium in the explants, but the reverse was not true. Finally, the relative growth rate and relative performance of the explants, under the combined effect of B and NaCl, showed a rapid decline at high boron and salt treatment.

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