Effects of Bone Marrow Stromal Cells (BMSCs) on Behavior, Infarct Size and HIF-1αExpression in Stroke Rats

Abstract

This study aims to assess BMSCs’ effect on the behavior, infarct size and HIF-1αexpression in stroke rats. Rats were separated into sham group, CVA group and BMSCs group with 10 rats in each group followed by analysis of neuroethology scores, brain tissue pathology and infarct size, and HIF-1αlevel in brain tissues. No difference of neurological scores was found between CVA group and BMSCs group after 3 hours (P> 0.05). After BMSCs transplantation, the nerve score was significantly reduced (P< 0.05) and cognitive function was significantly improved compared to CVA group. Compared with sham rats, CAV rats had a larger area of infarction and the infarcted tissue cells showed degeneration or necrosis with reduced cell number and obvious edema, which were all improved in BMSCs group. CVA group showed a larger area of infarct tissue (P< 0.05), which was reduced in BMSCs group (P< 0.05). Compared with sham group, CVA group showed significantly upregulated HIF-1αlevel (P< 0.05) which was reduced in BMSCs group (P< 0.05). BMSCs has a certain repair effect on the ethology of stroke rats possibly via inhibition of HIF-1αlevel in cerebral infarction and brain tissue.