Effects of bok choy on the dissipation of dibutyl phthalate (DBP) in mollisol and its possible mechanisms of biochemistry and microorganisms

Abstract

Phytoremediation is an ecologically sustainable method for remediating contaminated soils, however, research on phytoremediation and its mechanisms are still rarely reported. The highest dibutyl phthalate (DBP) dissipation rate was 91% in 0–3mm bok choy rhizosphere via a 45-day rhizo-box experiment, and bok choy could regulate soil nutrients by increasing soil ammonia nitrogen (AN) and available phosphorus (AP). The biochemistry mechanism of interaction between dissolved organic matter (DOM) and DBP was also elucidated by various spectroscopy techniques. It was found that the alkyl ester in DBP produced the fastest response during the binding process, and the aromatic, hydroxyl and phenolic groups of the DOM humic-like substances preceded amide in DOM protein-like substance. It was found that DBP pollution reduced the Chao1 richness and Shannon index of bacteria in black soil via a pot experiment and high-throughput sequencing, which disturbed the metabolic activities and functional diversity of microorganisms in Mollisol. The microbial abundance increased in bok choy amendments, which has a specific microbial community structure and a high abundance of Actinobacteria and Acidobacteria. We concluded that some enriched genera were responsible for DBP dissipation, Alsobacter, Lacibacter, Myceligenerans, Schrenkiella parvula and Undibacterium. The findings of this study revealed that the possible biochemistry and microbial mechanisms of phytoremediation promoting the DBP dissipation in rhizosphere Mollisol and provided more useful information for phytoremediation of organic pollutants.