Effects of BMP-2 Delivery in Calcium Phosphate Bone Graft Materials with Different Compositions on Bone Regeneration.

Jin-Chul Park,Ji-Hyeon Bae,Jae-Won Choi,Jae-Jun Ryu,Eun-Bin Bae,Se-Eun Kim,Kyung-Hee Choi,Jung-Bo Huh,So-Yun Kim

doi:10.3390/ma9110954

Abstract

This study was undertaken to investigate the effect of loading rhBMP-2 onto biphasic calcium phosphate (BCP) and calcium pyrophosphate (CPP) on bone regeneration, and to examine the efficacies of BCP and CPP as rhBMP-2 carriers. Specimens were divided into the BCP, CPP, BCP/BMP, and CPP/BMP groups; BCP and CPP were in granules and not coated with rhBMP-2. BCP/BMP and CPP/BMP were prepared as discs, which were treated with rhBMP-2 and collagen. Physical and biological features were investigated using in-vitro and in-vivo tests. New bone area percentages (%) in the BCP/BMP and CPP/BMP groups were significantly greater than in the BCP and CPP groups. At weeks 4 and 8 post-implantation, CPP/BMP showed the most new bone growth. Within the limitations of this study, treatment of BCP and CPP with rhBMP-2 significantly enhanced bone regeneration. CPP was found to be a suitable carrier for rhBMP-2.

Highlights

The use of biomaterials for bone regeneration has been widely utilized by dental bone augmentation procedures such as extraction socket defect grafting, sinus augmentation, and ridge augmentation [1]
We investigated the effect of loading recombinant human bone morphogenetic protein-2 (rhBMP-2) into calcium phosphate bone graft materials with different compositions (BCP and calcium pyrophosphate (CPP)) on bone regeneration and evaluated the feasibility of using biphasic calcium phosphate (BCP)
BCP had a pore size of 0.1 μm–1 μm (Figure 1e), whereas CPP had a pore size of 10 μm–100 μm (Figure 1f). rhBMP-2 with collagen covered the surfaces of BCP/BMP (Figure 1g) and CPP/BMP

Summary

Introduction

The use of biomaterials for bone regeneration has been widely utilized by dental bone augmentation procedures such as extraction socket defect grafting, sinus augmentation, and ridge augmentation [1]. Bone graft biomaterials should possess the properties of osteoconduction and osteoinduction to promote the formation of new bone [2]. Autogenic bone grafting has been traditionally used for defect reconstruction, but more recently a variety of graft materials, such as allogenic, xenogenic, and synthetic bones, have been introduced [3,4]. Autogenic bone is the most ideal graft material due to its outstanding biocompatibility, bone formation ability, osteoinductivity, and osteoconductivity. Allogenic and xenogenic bones are obtained from corpses and animals, respectively, and no harvesting is required and availability is not an issue, but immune rejection

Methods

Results

Conclusion