Effects of black cumin (Nigella sativa) oil on ammonia and biogenic amine production in rainbow trout

Abstract

In the present study, 0.00% (control), 0.10%, 0.40%, 0.70%, 1.00% and 1.30% black cumin oil was added to rainbow trout feed and the fish were fed for 120 days. Following the feeding period, the harvested fish were stored in the refrigerator at ±2 ºC, in Styrofoam boxes filled with ice, for 23 days. During the storage period, the effects of black cumin oil on ammonia and biogenic amine production in rainbow trout were examined. An increase was observed in the ammonia content during the storage period, with the most substantial increase observed in the control group and the least substantial increase observed in the 1.30% black cumin oil group. The biogenic amine level increased during the storage period. A significant increases was observed in the tyramine level during the storage period ( less than 3.08 mg/100 g), and significant differences were observed between the groups in terms of spermidine, spermine, histamine, and tyramine content (p less than 0.05). The results of the present study suggest that the addition of black cumin oil reduced ammonia and biogenic amine production in rainbow trout.