Effects of bioflocs with different C/N ratios on growth, immunological parameters, antioxidants and culture water quality in Opsariichthys kaopingensis Dybowski

Abstract

Biofloc technology (BFT) is a new ecological aquaculture technology that is intended not only to eradicate pollutants and elevate feed utilization but also to enhance immunity and antioxidant activity in aquatic animals. A 28-day feeding trial was implemented to evaluate the effects of promoted bioflocs on the water quality, growth performance, immunological parameters and antioxidant status of Opsariichthys kaopingensis Dybowski juveniles in low exchange culture tanks. Three hundred sixty healthy O. kaopingensis juveniles (7.1 ± 0.02 g) were irregularly distributed among 12 tanks. Four C:N ratios in triplicate tanks were tried: C/N = 10.8:1 with a commercial diet (control), C/N = 15:1, C/N = 20:1 and C/N = 25:1. Anhydrous glucose (99.97%) was added to the commercial diet to maintain elevated carbon ratio in the three treatments. Uninterrupted 24-hr aeration was supplied during the test by using an air-stone connected to an air pump to develop the bioflocs in the water column. The results indicated that BFT significantly reduced total ammonia nitrogen (TAN), nitrite (NO2−-N), total phosphorus (TP) concentrations and water transparency (Secchi depth) in C/N 15, C/N 20 and C/N 25 (p < .05), whereas the chemical oxygen demand (COD) and nitrate nitrogen (NO3−-N) concentrations gradually increased over time. In addition, weight gain, specific growth rate (SGR), survival and protein efficiency ratio (PER) were significantly elevated in C/N 20, while food conversion rate (FCR) was considerably decreased (p < .05) in C/N 20, compared with the control. Significant increases in lysozyme (LSZ), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and alkaline phosphatase (AKP) activities of O. kaopingensis were found in the C/N 20 group after a 28-day feeding trial (p < .05). Comparing the antioxidant capacity of O. kaopingensis in gills, brains, kidneys, hepatopancreas, intestines and serum of juveniles from the four experimental groups, the activity of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT) activity and glutathione peroxidase (GSH-PX) activity of juveniles in the C/N 20 group were significant higher (p < .05), and the content of malondialdehyde (MDA) was considerably lower, than in the control. Overall, these findings suggest that BFT not only can enhance O. kaopingensis growth performance and strengthen antioxidant status but also can upgrade immune response, ensuring the sustainable development of aquaculture.