Effects of biochar particle size and concomitant nitrogen fertilization on soil microbial community structure during the maize seedling stage.

Abstract

Biochar is widely used as a soil amendment, either alone or in association with fertilizer. However, the effects of biochar particle size on the soil microbial community are largely unclear. Biochar was divided into two groups according to diameter sizes: < 1mm and 2.5-5mm (labeled as CB1 and CB5, respectively). A pot experiment was established in which maize (Zea maize L.) was treated with CB1, CB5, and/or external nitrogen (N, NH4NO3). At the end of the seedling period (45days), analyses of soil microbial community structure and other soil and crop properties were conducted. The biochar (regardless of N addition) enhanced microbial biomass and activity. CB1 had a stronger capacity than CB5 to modify soil microbial community structure by promoting soil microbial groups (e.g., fungi, Gram-negative bacteria), which is likely due to CB1 undergoing a series of more intense processes (e.g., nutrient release, mineralization) than CB5. However, this difference was diminished or disappeared when N was added, mainly due to the masking effect of soil acidification that was induced by N fertilization. Collectively, fine biochar has a stronger effect on soil microbial community than coarse biochar. Particle size only affects soil microbial community structure when biochar is applied alone; it has no effect when biochar is applied in association with chemical N fertilizer, at least during the seedling period. The relationship between particle size and soil microbial community needs to be considered when using biochar for soil amendment.