Effects of biochar on soil microbial community and functional genes of a landfill cover three years after ecological restoration

Abstract

Landfills, as the most common approach to disposing of municipal solid waste worldwide, disturb native ecosystems and create a need for ecological restoration. The restoration of landfill cover with biochar has shown immediate potential to improve soil microbial functions within one year. However, such characteristics could change after a longer period of time. Here, soil properties, microbial communities, and microbial functional genes related to nutrient cycling were investigated three years after the biochar amendment of the topsoil of a subtropical landfill cover. The results showed that the levels of soil organic matter, water content, total carbon (C), total nitrogen (N) and total phosphorus (P) of biochar-amended soils were higher than those of control soil. Different soil microbial community structures were observed in the biochar-amended and control soils. Nine phyla, including Proteobacteria and Acidobacteria, but not Actinobacteria or Chloroflexi, were enriched in the biochar-amended soil. Although the impact of biochar on shaping microbial communities increased after a longer period of restoration, no differences were observed in soils that were amended using different biochar:soil ratios. The abundances of functional genes related to C and N cycling decreased, whereas those of genes related to P cycling were higher in soils that received biochar amendment. This finding suggests that compared with the control soil, biochar-amended soils were less active in processes involved in C and N cycling but enhanced in processes related to P cycling. This study can serve as a reference for future ecological restoration of degraded lands using biochar.