Effects of bile acids on aflatoxin B1 bioaccumulation, detoxification system, and growth performance of Pacific white shrimp

Abstract

The potential of bile acids (BAs) to reduce aflatoxin B1 (AFB1) residues and toxicity in Litopenaeus vannamei was evaluated. Both juveniles and subadults were treated with 0, 0.05, 0.15 and 0.25 g/kg BAs for 60 days followed by 10-d AFB1 exposure (2000 μg/kg), and fifteen shrimp (five shrimp were pooled into one sample, n = 3) from each treatment were collected at five time points (30, 60, 63, 66 and 70 d). All parameters were determined using accepted and standard methods with acceptable accuracy (recovery) of 90–110%. Results demonstrated that BAs reduced the AFB1 residues in shrimp (limit of detection: 0.01 μg/L, relative standard deviation < 10% and recovery: 92.1–96.8%). BAs increased the detoxification of AFB1 and decreased the levels of oxidative stress products by increasing Phase II and antioxidant systems, avoiding AFB1-induced deterioration of shrimp meat and health risks to human. The confidence level was 95%.