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Abstract
Tannic acid (TA) and TA containing beverage have been proved to inhibit Ca2+-activated Cl- channel located apical membrane of the secretory cells. However, their effect on salivary fluid secretion is not well investigated. We used mouse ex Vivo submandibular gland perfusion technique to identify the general effect of TA and related beverage samples on muscarinic agonist carbachol induced fluid secretion. Green tea inhibited fluid secretion by 64% from the control, where oolong tea was by 53%, and red wine by 43% which was linked with their TA concentration. On the other hand, though TA was contained at 4.7 μM in white wine sample and 33 μM in coffee extract, no adverse effect was observed. In addition, coffee induced salivation in the absence of carbachol. TA had a negative effect on fluid secretion with a concentration dependent manner. The effects of TA on carbachol induced calcium increase showed identical as fluid secretion, which was initially no effect, and then gradually decreased over the time. These results demonstrate that TA directly inhibits the salivary fluid secretion and it affects not only Ca2+-activated Cl- channel but also intracellular Ca2+ increasing mechanisms.
Highlights
Saliva is one of the most important components in the oral cavity, which helps taste sensation, speech, mastication, swallowing and protects oral mucosa and teeth [1] [2]
Salivary glands consists two components: one is acinar cells where isotonic fluid is made; and the other is ductal cells where absorb most of NaCl, and add K+ and HCO3− in original saliva, and final saliva become hypotonic [3]
Transcellular anion transport especially in salivary acinar cells has a key role for regulating the fluid secretion; these are mainly composed of two anion inlet transporters in basolateral membrane and outlet in apical membrane
Summary
Saliva is one of the most important components in the oral cavity, which helps taste sensation, speech, mastication, swallowing and protects oral mucosa and teeth [1] [2] It is originally made from the blood, the most of it in three major salivary glands, parotid, submandibular and sublingual glands. Transcellular anion transport especially in salivary acinar cells has a key role for regulating the fluid (water) secretion; these are mainly composed of two anion inlet transporters in basolateral membrane and outlet in apical membrane. The former one is mainly composed of Na+-K+-2Cl− cotransport [4] and partially of anion exchangers working simultaneously Na+/H+ exchangers [5]. We used a biological sensor—ex vivo mouse submandibular gland perfusion technique—to identify the effect of the TA including beverage ingredients on the glandular function
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