Abstract
Addition of increasing amounts of benzyl alcohol progressively reduced the steady-state anisotropies of diphenylhexatriene and trimethylammoniumdiphenylhexatriene in brush-border membranes from rat kidney. The decrease in order of membrane lipids, equivalent for 50 mM benzyl alcohol to that produced by a rise in temperature of approx. 6°C, had no effect on the activities of alkaline phosphatase or γ-glutamyltranspeptidase. On the other hand, benzyl alcohol markedly inhibited the d-glucose uptakes measured in the presence of a 100 mM sodium gradient. For concentrations less than 30 mM, benzyl alcohol reduced the J max without significant effects on K m, 22Na + uptake or the vesicular volume of brush-border preparations. Comparable results were obtained substituting octanol for benzyl alcohol. Our data strongly suggest that, at constant temperature, the d-glucose carrier present in renal brush-border membranes is extremely sensitive to variations in membrane physical state.
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