Effects of baicalin on c-myc expression in HL-60 cells

Abstract

Objective To investigate the effects of baicalin on proliferation inhibition and apoptosis induction in human myeloid leukemia cell line HL-60 cells and on c-myc gene and protein expressions. Methods HL-60 cells were exposed to haicalin at different dosages. Proliferation inhibition was detected by MTT assay. The ability of haicalin to induce apoptosis of HL-60 cells was examined by DNA fragmentation. The expression of c-myc was detected by RT-PCR and Western-blot. Results Baicalin remarkably inhibited the HL-60 cells proliferation, with an IC50 value of 20 μmol/L. Concomitantly, apoptosis was induced in HL- 60 cells, as measured by detection of DNA fragmentation. The expressions of c-myc in HL-60 induced by haicalin decreased. Conclusion Baicalin could efficiently induce proliferation inhibition and apoptosis in HL-60 cells, c-myc may involved in these processes. Key words: Baicalin; Apoptosis; Human myeloid leukemia cell line HL-60 cells; c-myc gene expressions