Abstract
Bacterial lipopolysaccharide (LPS)-induced colony-stimulating activity (CSA) in murine long-term bone marrow culture system was investigated. Bone marrow culture cells of LPS-nonresponsive C3H/HeJ mice responded to LPS in terms of CSA production as efficiently as bone marrow culture cells of LPS-responsive C3H/slc mice. On the other hand, both peritoneal macrophages and bone marrow macrophages from C3H/HeJ mice did not produce CSA in vitro after treatment with LPS. Percoll density gradient separation of adherent layer cells in bone marrow cultures showed that two cell populations were present. One population was nonspecific esterase positive, productive of high CSA to LPS stimulation and light density cells, the other population was nonspecific esterase negative, productive of low CSA to LPS stimulation and high density cells, and CSA production stimulated by LPS in C3H/HeJ mice bone marrow culture cells was mainly attributed to the latter population of cells. These results suggest that CSA production stimulated by LPS in C3H/HeJ mice is regulated by different cell populations, respectively in vivo and in vitro.
Published Version
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