Abstract
The effects of bacteria-produced human interferons (HuIFN) α, β, and γ on in vitro immune functions of human peripheral blood mononuclear cells (PBMC) were studied. Proliferative response to phytohemagglutinin was significantly inhibited by the addition of HuIFN-α2 or HuIFN-β at 10, 100, or 1000 U/ml. In contrast, HuIFN-γ showed suppressive activities only when added at 1000 U/ml. HuIFN-α2 or HuIFN-β caused significant inhibition of human mixed-lymphocyte reaction (MLR) as measured by [ 3H]thymidine incorporation. Similar inhibition was caused by HuIFN-γ when it was added only at very low concentrations (1 U/ml); 10, 100, or 1000 U/ml resulted in no or only a modest increase in MLR. All three interferons exhibited dose-related effects on PWM-induced immunoglobulin synthesis in cultures of PBMC. These data demonstrate that purified interferons produced by recombinant DNA technology can significantly alter in vitro immune functions and that HuIFN-γ has properties which are different from those of HuIFN-α2 or HuIFN-β.
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