Effects of Auxin and Gibberellins Hormones on Regeneration of Pineapple Propagules from Crown Leaves (with Buds) in Kilifi County Kenya

Abstract

Kilifi county has a large potential of over 2,000 Ha suitable for pineapple cultivation that has not been fully exploited due to lack of adequate clean planting material. Thus, the aim of the study was to evaluate the effects of auxin and gibberellin hormones on regeneration of pineapple propagules from pineapple crown leaves (with buds). The study was carried out at Pwani University Crop Science farm. A randomized complete block design with split plot arrangement replicated thrice was used. Treatments included auxin and gibberellins hormones where the cut crown leaves (with buds) were dipped and planted at a spacing of 4 cm by 6 cm in plastic containers filled with coir dust. Five plastic containers were used for each hormone treatment. In each container, three plants were tagged for data collection. The plastic containers (the plots) measured 30cm by 36cm by 15cm height. Data collected from the tagged plants included, time to shoot emergence, plant height, leaf length and breadth, number of leaves per plant, final root length and final number of roots. These were used to determine effects of auxin, gibberellins on growth of the propagules up to a height of 15-20 cm. Obtained data was subjected to ANOVA analysis using Genstat Statistical software 14th Edition and means obtained compared using Tukey’s honest significant difference test (p < 0.05). The results indicated auxin treated propagules had 50.7%, 50.6%, 64.2%, 52.6%, 24.4%, 10.4%, 39.0%, and 9.9% higher final values of plant weight, shoot weight, dry shoot weight, fresh root weight, plant height, leaf number, root number, dry root weight and root length, respectively, compared to those treated with gibberellins. The study also showed that auxin treated propagules had their initial roots originating from the base of the seedlings, while gibberellins treated propagules had their initial roots originating from the base of the cut crown leaves.