Abstract
Purpose To explore the role of all-trans retinoic acid (ATRA) in renal ischemia/reperfusion injury of diabetic rats.Methods Sixty adult male rats were randomly divided into 6 groups, including sham group (S group), ischemia-reperfusion group (I/R group), ischemia-reperfusion+ATRA group (A group), diabetic group (D group), diabetic ischemia-reperfusion group (DI/R group), diabetic ischemia-reperfusion +ATRA group (DA group). The levels of creatinine (Cr), cystatin C (Cys-C) and β2-microglobulin (β2-MG) were measured. Morphology of renal tissue was observed under light microscope.Results DJ-1, Nrf2, HO-1 and caspase-3 were detected by western blot. DJ-1, Nrf2, HO-1 and caspase-3 in I/R group, D group and DI/R group was higher than that in S group. Compared with I/R group, Nrf2 and HO-1 in A group was decreased, but caspase-3 was increased. However, Nrf2 in DA group was higher than that in DI/R group, HO-1 and caspase-3 in DA group were lower than that in DI/R group. Compared with group S, Cr, Cys-C and β2-MG in I/R group, A group, D group, and DI/R group were higher. Whereas the levels of Cr, Cys-C, β2-MG and renal injury score in DA group were lower than those in DI/R group.Conclusion ATRA has a protective effect on renal ischemia-reperfusion injury in diabetic rats, maybe relating to DJ/Nrf2 pathway.
Highlights
Diabetes mellitus, as a chronic metabolic disorder, is characterized by an abnormal insulin secretion and action resulting from interaction of hereditary and environmental factors
This study aimed to investigate whether All-trans retinoic acid (ATRA) had a protective effect on renal ischemia- reperfusion in diabetic rats and whether it was related to DJ-1/Nrf[2] pathway
Compared with the renal histological evaluation score of kidneys obtained from sham group (S group), I/R group and diabetic group (D group) exhibited a clearly increase in renal histologic evaluation score (P
Summary
As a chronic metabolic disorder, is characterized by an abnormal insulin secretion and action resulting from interaction of hereditary and environmental factors. As one of the common complications of diabetes, diabetic nephropathy has an increasing gradually incidence. The pathogenesis of diabetic nephropathy is associated with the interaction of hyperglycemia, advanced glycation end-product, increased po-lyol pathway and oxidative stress[1]. Renal ischemia-reperfusion injury occurs in diabetic patients, which aggravates diabetic nephropathy. Studies have shown that ATRA can effectively prevent the progression of diabetes in rats. Pancreatic β-cells, acinar and ductal cells gradually restore their normal appearance under ATRA treatment. What’s more, insulin messenger RNA and serum indices almost normalize, which improves the histological changes of the pancreas and the serum indices in diabetic rats[3,4]. ATRA can affect the progression of diabetic nephropathy without causing any significant side effects and has a therapeutic effect on diabetic nephropathy[5]
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