Abstract
The polysaccharide is the main active substance contained in Hericium erinaceus and is commonly used in the treatment of neurasthenia, tumors, and digestive diseases. Six intracellular polysaccharide components were obtained from H. erinaceus fruiting bodies cultivated by ARTP (atmospheric and room temperature plasma) mutagenic strain (321) and the original strain (0605), respectively. This study was designed to investigate the physicochemical characteristics of these polysaccharide components and their potential immunomodulatory activities on RAW264.7 macrophages. The results showed that the yield of fruiting body cultivated by mutated strain increased by 22% and the polysaccharide content improved by 16% compared with the original one owing to ARTP mutagenesis. The molecular weight distribution and the monosaccharide compositions of polysaccharide components from H. erinaceus induced by ARTP mutagenesis were significantly different from that of the original one. The NO, IL-6, IL-10, IL-1β, and TNF-α production activities of macrophages were enhanced by stimulation of 20% ethanol precipitated polysaccharides from H. erinaceus induced by ARTP mutagenesis. These results indicated that ARTP is an efficient and practical method for high polysaccharide content breeding of the H. erinaceus strain and this provided a reference for obtaining high quality resources and healthy product development from H. erinaceus.
Highlights
Hericium erinaceus (H. erinaceus) is commonly consumed as edible or medicinal sources in China and other oriental countries and is known as lion’s mane mushroom or hedgehog mushroom [1,2].Previous studies indicated H. erinaceus has great medicinal value due to its various beneficial effects, especially for gastrotherapy [3,4]
The mutagenic strain 321, which screened from hundreds of strains bred through Atmospheric pressure room temperature plasma (ARTP), had been identified by an antagonism test and random amplification polymorphic DNA (RAPD) analysis with changes in genetic materialmaterial with thewith parent
The results further indicated that the molecular weight, monosaccharide composition, and other structural characteristics of H. erinaceus polysaccharides were related to their immune activity in vitro
Summary
Hericium erinaceus (H. erinaceus) is commonly consumed as edible or medicinal sources in China and other oriental countries and is known as lion’s mane mushroom or hedgehog mushroom [1,2].Previous studies indicated H. erinaceus has great medicinal value due to its various beneficial effects, especially for gastrotherapy [3,4]. Many bioactive compounds have been isolated and identified from H. erinaceus, such as hericenones, erinacines, glycoprotein, polysaccharides, steroids, alkaloids, and hericins [5,6,7]. Among these compounds, polysaccharides isolated from its fruiting bodies are Molecules 2019, 24, 262; doi:10.3390/molecules24020262 www.mdpi.com/journal/molecules. A number of functional foods and medicines made from H. erinaceus fruiting bodies have appeared on the Chinese market, which contain polysaccharides as the main functional ingredient [9]. Changes in the structural properties and activities of polysaccharides improved by mutagenesis has not been reported
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