Abstract
Inbred mouse strains C57BL/6J (B6) (susceptible) and C3H/HeJ (C3H) (resistant) differ in atherosclerosis susceptibility due to a single gene, Ath-1. Plasma lipoproteins from female mice fed chow or an atherogenic diet displayed strain differences in lipoprotein particle sizes and apolipoprotein (apo) composition. High density lipoprotein (HDL) particle sizes were 9.5 +/- 0.1 nm for B6 and 10.2 +/- 0.1 nm for C3H. No major HDL particle size subclasses were observed. Plasma HDL level in the B6 strain was reduced by the atherogenic diet consumption while the HDL level in the resistant C3H mice was unaffected. The reduction in HDL in the B6 strain was associated with decreases in HDL apolipoproteins A-I(-34%) and A-II(-60%). The HDL apoC content in mice fed chow was two-fold higher in C3H than B6. Lipoproteins containing apolipoprotein B (VLDL, IDL, LDL) shifted from a preponderance of the B-100 (chow diet) to a preponderance of the B-48 (atherogenic diet). The LDL-particle size distribution was strain-specific with the chow diet but not genetically associated with the Ath-1 gene. In both strains on each diet, apolipoprotein E was largely distributed in the VLDL, LDL, and HDL fractions. The B6 strain became sixfold elevated in total lipoprotein E content which in the C3H strain was not significantly affected by diet. However, the C3H LDL apoE content was reduced. On both diets, the C3H strain exhibited apolipoprotein E levels comparable to the atherogenic diet-induced levels of the B6 mice.
Highlights
H D L particle size subclasses were observed
In the between strains C57BL/6 (B6) strain, consumption of the atherogenic diet resulted in the following major changes in lipoprotein profiles when compared to profiles from mice fed the chow diet: I ) a single, major peak appeared in the VLDL-LDL-size region; and 2) the peak area in the High density lipoprotein (HDL)-size region was reduced approximately
We describe changes in the apolipoprotein composition of plasma lipoproteins from the C3H and B6 mouse strains after prolonged consumption of an atherogenic diet
Summary
Plasma H D L level in the B6 strain was reduced by the atherogenic diet consumption while the HDL level in the resistant C3H mice was unaffected. The LDL-particle size distribution was strainspecific with the chow diet but not genetically associated with the Ath-1 gene. In both strains on each diet, apolipoprotein E was largely distributed in the VLDL, LDL, and HDL fractions. Atherogenic diet consumption results in plasma HDL-C levels of 31 6 mg/dl (mean f SEM) in the susceptible B6 strain (a twofold reduction) and an unchanged level of 62 5 8 mg/dl in the resistant C3H strain [4]. In this report we compare four lipoprotein classes isolated by ultracentrifugation using GGE to determine the particle size and relative quantities of lipoprotein, and quantitative SDSPAGE to determine the apolipoprotein composition of each lipoprotein class
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