Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFV in vitro.

Zengyu Zhuge,Yanpeng Dong,Liuan Li,Tianming Jin

doi:10.7717/peerj.3862

Abstract

BackgroundAstragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown.MethodsThe virus was titrated using an indirect immune biotin enzyme standard method to confirm that porcine ECs were susceptible to CSFV infection and to determine the TCID50 of CSFV (C-strain). Porcine ECs were cultured with CSFV in the presence of APS. Relative quantitative PCR was used to assess the mRNA expression of factors that influence EC adhesion and immunity.ResultsThe expression of adhesion factors mRNA increased following stimulation with CSFV; this effect was inhibited by pre-exposing the cells to APS. In addition, the expression of growth factors and some immune factors increased after infection with CSFV; this increase in tissue factor (TF), transforming growth factor (TGF-β), and interleukin-8 (IL-8) could be inhibited by the addition of APS. The immune response mediated by Toll-like receptor 4 (TLR4) in ECs may be unregulated by CSFV as it was also inhibited by pre-treatment with APS.DiscussionThe addition of APS to the culture can obviously regulate the expression of molecules related to the adhesion, growth, and immune response of ECs, as well as the production of cytokines. Therefore, it may have the potential to be an effective component in vaccines against CSFV.

Highlights

Classical swine fever (CSF) is a highly contagious disease affecting swine and leads to tremendous economic losses worldwide
After 3 h of stimulation with classical swine fever virus (CSFV) (Fig. 2B), a significant decrease in the mRNA expression of P-selectin (P = 0.001) compared to medium alone was detected in Groups 2 and 3, which were treated with Astragalus polysaccharide (APS)
At 6 h post-stimulation, the mRNA expression of P-selectin was significantly increased in the groups stimulated with CSFV (Groups 4, 5, and 6) compared with groups that were not stimulated with CSFV (Groups 1, Figure 2 The relative mRNA expression of P-selectin (A), E-selectin (B), vascular endothelial growth factor (VEGF) (C) and tissue factor (TF) (D) in porcine endothelial cells (ECs)

Summary

Introduction

Classical swine fever (CSF) is a highly contagious disease affecting swine and leads to tremendous economic losses worldwide. How to cite this article Zhuge et al (2017), Effects of astragalus polysaccharide on the adhesion-related immune response of endothelial cells stimulated with CSFV in vitro. Astragalus polysaccharide (APS) has immunomodulatory activities on porcine peripheral blood mononuclear cells. The immunomodulatory effects of APS on porcine endothelial cells (ECs) expose to classical swine fever virus (CSFV) remain unknown. The virus was titrated using an indirect immune biotin enzyme standard method to confirm that porcine ECs were susceptible to CSFV infection and to determine the TCID50 of CSFV (C-strain). The expression of adhesion factors mRNA increased following stimulation with CSFV; this effect was inhibited by pre-exposing the cells to APS. The addition of APS to the culture can obviously regulate the expression of molecules related to the adhesion, growth, and immune response of ECs, as well as the production of cytokines. It may have the potential to be an effective component in vaccines against CSFV

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Conclusion