Abstract
The present work is the first attempt to study the effect of such assisted reproductive technologies (ARTs), as in vitro culture of preimplantation embryos on the social behavior of offspring, using BTBR mice (BTBR T+Itpr3tf/J) as an idiopathic model of a-utism. The C57BL/6J mice were used as controls. Social behavior was studied in adult offspring mice obtained after in vitro culture and embryo transfer (ET) (groups ET-C57BL/6J and ET-BTBR). The BTBR mice demonstrated the reduced levels of social recognition and affiliation compared to C57BL/6J mice. The social affiliation and recognition test revealed that ET-C57BL/6J males had a high level of social interaction, which was of similar magnitude as in naturally conceived C57BL/6J males. However, ET-C57BL/6J and ET-BTBR females demonstrated a decrease in interest to a new stranger compared to naturally conceived controls of the same sex, which was revealed in a social recognition session of this test. Thus, the in vitro culture affected the social recognition in the offspring of C57BL/6J and BTBR mice, but only in females.
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