Effects of aspirin on mesenteric lymph nodes of rabbits as basis for its use on lymph nodes metastases

Rodrigo Peduti Batista,Rafael Denadai,Rogério Saad-Hossne

doi:10.1590/s0102-86502012001100009

Abstract

To evaluate the effects of aspirin 10% and 20% on mesenteric lymph nodes of rabbits as basis for its use on lymph nodes metastases. A total of 20 lymph nodes from 20 rabbits (randomized in four groups) were evaluated. Aspirin solutions 10% (groups A and C) and 20% (groups B and D) were injected into mesenteric lymph nodes of healthy rabbits and had its gross and histological effects evaluated at 24 hours (groups A and B) and at seven days (groups C and D). In the groups A and B evaluated at 24 hours it was observed extensive necrosis and hemorrhage, a significant increase in apoptosis throughout the lymph node with medullary sinuses enlargement and an increase in germinal centers. In the groups C and D evaluated at seven days of solution injection there was also an increase in apoptosis with higher elevation of histiocytes and a significant decrease of necrosis and an increase of giant cells was noticed causing a foreign body chronic inflammation. In all comparisons, there were no differences between the concentrations used (10 and 20%). The injection of aspirin on lymph nodes caused necrosis and an increase of apoptosis after 24 hours and after seven days of treatment there was regeneration of the lymph nodes, with intense decrease of necrosis and a great elevation of apoptosis. These experimental results support future clinical studies on application of aspirin in the treatment of lymphatic metastases, since the increase of apoptosis is one of the pillars of cancer therapy.

Highlights

Cancer is the leading cause of death in economically developed countries and the second leading cause of death in developing countries[1]
Once a large number of patients have distant metastases on lymph nodes adjacent to the tumor at initial diagnosis and the lymph node spread is a major determinant for the staging and the prognosis of most human malignancies[2], their treatment is needed
We had the opportunity to study and scientifically prove cytolytic effects of aspirin and its derivatives, either in vitro or in vivo on several experimental models[6,7,8,9,10]. Following this line of research, the purpose of this experimental study was to evaluate the effects of aspirin on normal lymph nodes of rabbits as basis for its later use on lymph nodes affected by tumor

Summary

Introduction

Cancer is the leading cause of death in economically developed countries and the second leading cause of death in developing countries[1]. Resection surgery is considered the main type of treatment for cure of lymphatic metastases but in some situations it presents limiting factors such as number and location of lymph nodes involved and especially the adherence to adjacent organs and structures[3,4]. Considering such difficulties and therapeutic characteristics of lymphatic metastases[3,4], new treatment options need to be developed[5]. Following this line of research, the purpose of this experimental study was to evaluate the effects of aspirin on normal lymph nodes of rabbits as basis for its later use on lymph nodes affected by tumor

Methods

Results

Findings

Discussion

Conclusions