Effects of Asian Dust Particles on the Early-Stage Antigen-Induced Immune Response of Asthma in NC/Nga Mice.

Jun Kurai,Eiji Shimizu,Masanari Watanabe,Yuji Tohda,Degejirihu Hantan,Hiroyuki Sano

doi:10.3390/ijerph13111144

Jun Kurai, Eiji Shimizu + Show 4 more

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https://doi.org/10.3390/ijerph13111144

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Abstract

Asian dust (AD) can aggravate airway inflammation in asthma, but the association between AD and the development of asthma remains unclear. This study aimed to investigate the effects of AD on the early stage of antigen sensitization using a mouse model of asthma, as well as the role of leukotrienes (LTs) in antigen-induced airway inflammation potentiated by AD particles. NC/Nga mice were co-sensitized by intranasal instillation of AD particles and/or Dermatophagoides farinae (Df) for five consecutive days. Df-sensitized mice were stimulated with an intranasal Df challenge at seven days. Mice were treated with the type 1 cysteinyl LT (CysLT1) receptor antagonist orally 4 h before and 1 h after the allergen challenge. At 24 h post-challenge, the differential leukocyte count, inflammatory cytokines, and LTs in bronchoalveolar lavage fluid were assessed, and airway inflammation was evaluated histopathologically. AD augmented neutrophilic and eosinophilic airway inflammation with increased CysLTs and dihydroxy-LT in a mouse model of asthma. The CysLT1 receptor antagonist was shown to attenuate both neutrophilic and eosinophilic airway inflammation augmented by AD. Therefore, exposure to AD may be associated with the development of asthma and LTs may play important roles in airway inflammation augmented by AD.

Highlights

The health burden of airborne particulate matter (PM) is an important environmental health concern, and a number of studies have demonstrated that airborne PM exposure can increase respiratory and cardiovascular morbidity and mortality [1,2]
This study investigated whether a CysLT1 receptor antagonist could abolish these initial immune responses augmented by Asian dust (AD) particles
Enhancement of airway inflammation compared with Dermatophagoides farina (Df)/Df mice, and the total cell count was

Summary

Introduction

The health burden of airborne particulate matter (PM) is an important environmental health concern, and a number of studies have demonstrated that airborne PM exposure can increase respiratory and cardiovascular morbidity and mortality [1,2]. Desert sand dust particles are an important component of airborne PM and can have great effects on health over large distances because they can travel far from their source [3,4]. Numerous epidemiological studies have found that AD emissions have effects on respiratory and cardiovascular morbidity and mortality [3,8]. The role of exposure to airborne PM in the development of asthma remains unclear. Several studies have found that exposure to airborne PM early in life may contribute

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