Effects of Aqueous Extract of Diospyros Kaki Calyx on Anti-thrombotic Activity in vitro and in vivo

Abstract

ABSTRACT Objectives：The aim of this study is to research an anti-thrombus effect by Diospyros Kaki Calyx.Methods：The healthy human plasma were gained and used in vitro study such as factor X activity (FXa) inhibition, prothrombinase inhibition, prothrombin time (PT) an d activated partial thromboplastin time. Fifteen SD rats were divided into three groups；intact control group (or ally administrated with distilled water 5ml/kg) and two experimental group treated with extract of diospyros kaki calyx (EKC). Experimental rats were orally 600 mg/kg concentration of EKC and 200 mg/kg concentration of EKC. After an hour from administration, we anesthetized rats and made arteriovenous (AV) shunt rat models to study weight of thrombus, took whole blood to study content of thromboxane B2 and blood clotting time.Results：In vitro, EKC significantly increased inhibitory activity of FXa, prothrombinase compared with intact control group (*P<0.05). PT and aPTT were increased in EKC trea ted (600 mg/kg) group compared with intact control group (*P<0.05). In vivo, blood clotting time of experiment group treated with EKC 600 mg/kg were significantly increased compare with that of intact control group (p<0.05) and content of thromboxane B2 was significantly decreased in group treated with EKC 600 mg/kg in serum. The weight of thrombus were significantly reduced in group treated with EKC 600 mg/kg compa red with intact control group (p<0.05). But in vivo experiment study, those parameters of group treated with EKC 200 mg/kg were relatively decreased compared with those of intact control group without statistical significance.Conclusions：EKC has an antithrombic activity because of inhibition internal course such as FXa and prothrombin. And EKC inhibited a hole blood clotting in vivo experiment by low content of thromboxane B2.Key words：Diospyros Kaki Calyx, antithromboic activity, factor Xa assay, prothrombin time assay, thromboxane B2, arteriovenous shunt modle