Effects of apoplastic sucrose on carbohydrate pools and sucrose efflux of mesophyll protoplasts of pea (Pisum sativum)

Abstract

The effects of the apoplastic, i.e. external, concentration of sucrose (0–30 mM) on O2 evolution, O2 consumption, starch, sucrose, glucose and fructose content, and uptake and efflux of sucrose in mesophyll protoplasts of Pisum sativum L. cv. Fenomen were studied. Neither photosynthesis, dark respiration, sucrose nor starch content change with increased apoplastic sucrose concentration. The contents of glucose and fructose in the protoplasts increase with increased apoplastic sucrose concentration. The sucrose efflux increases with increased external sucrose concentrations between 1 and 5 mM, but above this range the efflux decreases with increased external sucrose concentrations. These findings indicate that although external sucrose does not enter the protoplasts, there is a relationship between the external sucrose pool and the internal pools of sugars in the mesophyll protoplasts. The results suggest an active sucrose efflux from the protoplasts at physiological concentrations of apoplastic sucrose (max 5 mM) and a simple diffusion mechanism at higher concentrations.