Abstract
Objective To investigate the effects of Apollon antisense oligonucleotide (ASODN) on proliferation, apoptosis and drug resistance of human leukemia(K562) cells. Methods Specific phosphorothioate ASODN and missense oligonucleotide(MSODN) of Apollon mRNA were synthesized and transfected into K562 cells following cationic liposome.The proliferation inhibition of K562 cells was assessed by MTT.The apoptosis rate was detected by Annexin V-FITC.The sensitivity of K562 cells to etoposide and vincristine was detected by MTT. Results Apollon antisense oligonucleotide inhibition of K562 cells with the concentration and time increased.ASODN at a final concentration of 600 nmol/L could significantly inhibit the K562 cell proliferation.The apoptosis rate was apparently increased (P<0.01). Conclusions Apollon ASODN may decrease Apollon gene expression, suppress K562 cells proliferation effectively, and induce significant apoptosis of K562 cells.Apollon ASODN is able to reverse the drug resistance via inhibition of Apollon expression and inducement of apoptosis. Key words: Apollon; Antisense oligonucleotide; K562; Cell proliferation; Apoptosis; Drug resistance
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