Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells.

Masaaki Takechi,Mohammad Rahman,Yoshiaki Ninomiya,Kanji Tsuru,Akira Ohta,Kunio Ishikawa,Kazuki Sasaki,Misato Tada,Kouji Ohta

doi:10.3390/ma9040283

Masaaki Takechi, Mohammad Rahman + Show 7 more

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https://doi.org/10.3390/ma9040283

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Journal: Materials	Publication Date: Apr 13, 2016
Citations: 18	License type: CC BY 4.0

Affiliation: Hiroshima University, Kyushu University

Abstract

To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.

Highlights

Hydroxyapatite (HAP: Ca10 (PO4 )6 (OH)2 ) is widely used as a substitute material for the reconstruction of bone in oral maxillofacial surgery procedures [1,2]
We examined initial cell attachment, attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) cultured proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) cultured on the surface of on the surface of c-apatite cement (AC), AC(ate), as well as plastic cell culture wells used as a control
Containing atelocollagen and examined basic properties of AC with atelocollagen content from 0.5%–5%. They found that 2% content orthe over of properties of AC with atelocollagen content from 0.5%–5%

Summary

Introduction

Hydroxyapatite (HAP: Ca10 (PO4 ) (OH)2 ) is widely used as a substitute material for the reconstruction of bone in oral maxillofacial surgery procedures [1,2]. A disadvantage of sintered HAP is difficulty with complete fitting of the shape to the bone defect [3]. Apatite cement (AC) was developed to overcome this disadvantage, as it sets to form HAP after mixing of the powder phase and liquid phase [4,5,6]. Our previous report demonstrated that these three types of ACs have essentially the same levels of biocompatibility and osteoconductivity for proliferation and differentiation of osteoblasts in vivo [3]. Based on those findings, we considered that the ability of Materials 2016, 9, 283; doi:10.3390/ma9040283 www.mdpi.com/journal/materials these ACs

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