Effects of antisense oligodeoxynucleotides targeting hypoxia inducible factor 1α gene on huinan glioma cell line U87 in vitro

Abstract

Objective To investigate the effects of antisense oligodeoxynucleotides （ODNs） targe- ting hypoxia inducible factor-1α（HIF-1α） on the apoptosis, proliferation and invasion of U87 glioma cell line. Methods Antisense ODNs were constructed and transfected into U87 cells by Dosper liposomal reagent. The HIF-1α gene expression was detected by Western blotting, the cell proliferative index was determined by methyl thiazol tetrazolium （MTT） assay, the cells cycle and apoptosis of the cells were examined by flow cytometry and the changes of the U87 cells invasive ability were measured by Transwell chamber. Results The protein expression of HIF-1α in U87 ceils was down-regulated by HIF-1α ASONDN. The cell proliferative index in transfected group, empty vector group and control group was 5.46%, 21.25% and 22. 32% respectively. Transwell chamber assay showed that the cell number in transfected group, empty vector group and control group was （22 ±4）, （124±3） and （122 ±6） respectively; and the apoptosis rate was （53.35 ± 2.80） %, （ 12.02 ± 1.60） %, （ 10. 19 ± 3. 15 ） % respectively, and there was significant difference between transfected group and other groups （ P 〈 0. 05）. Conclusion Silencing the expression of HIF-1α protein can inhibit proliferation and invasion, and promote apoptosis of human glioma U87 cells. HIF-1α is expected to become a target for cancer therapy. Key words: Glioma; HIF-1α; Invasion; Proliferation