Abstract
Brown macroalgae (BM) which contain fucoxanthin exhibited high antioxidant activity. This study was performed to examine antioxidant, anti-collagenase, anti-elastase, anti-tyrosinase activities, and effect on cell viability of Human Dermal Fibroblast adult (HDFa) of BM, Turbinaria decurrens Bory. T. decurrens dried powder were macerated by ethanol to obtain extract (ETD) and was fractination by column chromatography to obtain fraction (FTD). Fucoxanthin content was determined using HPLC. The antioxidant activities, anti-collagenase, anti-elastase, and tyrosinase inhibitory assay were performed. The effect of ETD and fucoxanthin standard on cell viability were conducted on HDFa cell-induced by hydrogen peroxide (H2O2). The HPLC analysis showed that ETD and FTD contain fucoxanthin of 284.9±3.3µg/g and of 653.4±30.6µg/g dry-weight, respectively. The antioxidant assay showed that ETD and FTD produced high antioxidant activity by ferric reducing antioxidant power (FRAP) and b-carotene bleaching (BCB) methods that were comparable to fucoxanthin. ETD exhibited significantly higher tyrosinase inhibitory than kojic acid (p<0.01), while FTD had a comparable effect to kojic acid. The result also revealed that ETD and FTD produced anti-elastase and anti-collagenase (matrix metalloproteinase-1 (MMP-1). Fucoxanthin and ETD were able to maintain cell viability on HDFa cell-induced H2O2. This study suggests that T. decurrens may be effective to prevent skin aging and wrinkle formation, possibly through the antioxidant activity and maintain cell viability of fibroblast.
Highlights
The aging process is characterized by the gradual alteration of skin integrity and some physiological function (Farage et al, 2008)
The present study showed that fucoxanthin in ETD and FTD indicated that T. decurrens contained fucoxanthin and this finding consistent with the previous study of brown macroalgae (Zailanie and Sukoso, 2014; Jaswir et al, 2013)
Our findings demonstrated that extract (ETD) and fraction (FTD) of T. decurrens were able to inhibit MMP1 and human neutrophile elastase in vitro that indicating have an anti-wrinkle effect
Summary
The aging process is characterized by the gradual alteration of skin integrity and some physiological function (Farage et al, 2008). ROS such as superoxide anion radical, hydroxyl radicals, singlet oxygen, and hydrogen peroxide (H2O2) formed during normal metabolic processes, which can generate the lipid peroxidation and lead to the accumulation of lipid peroxides (Rinnerthaler et al, 2015) (Wondrak et al, 2006). The skin enzymes such as elastase and collagenase involve in the aging process and age. Body has several endogenous protection systems to diminishing excessive ROS Several antioxidants, such as ascorbic acid, tocopherols, carotenoids, natural substances, and polyphenols, should be effective to protect oxidative stress and prevent skin aging. The present study was performed to determine antioxidant activities, anti-collagenase, antielastase, anti-tyrosinase and the effect on human dermal fibroblast of T. decurrens Bory
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