Effects of antihypertensive drugs on expression of TRPC3 and TRPC6 in left ventricle of spontaneously hypertensive rats

Abstract

AIM: To investigate the effect of valsartan,ramipril and anlodipine on the expression of TRPC3 and TRPC6 in the left ventricle in spontaneously hypertensive rats(SHR).METHODS: Twelve-week-old SHRs were randomly divided into four groups: valsartan-treated group(Val),ramipril-treated group(Ram),amlodipine-treated group(Aml) and untreated group(SHR).Twelve-week-old Wistar-Kyoto(WKY) rats were used as control group.After 4 weeks of drug intervention,blood pressure(BP),left ventricular mass index(LVMI),left ventricular cardiomyocytes transection diameter(LVTDM) were examined,RT-PCR and Western blotting were used to detect the expressions of TRPC3 and TRPC6 mRNA and protein.RESULTS: Compared with those in WKY,BP,LVMI and LVTDM in SHR were significantly higher(P0.05) and the data mentioned above decreased significantly in the drug intervention groups(P0.05).Expressions of TRPC3 and TRPC6 mRNA and protein were detected in each group and were significantly higher compared with those in WKY.TRPC3 decreased dramatically in drug intervention groups(P0.05) and TRPC3 mRNA and protein decreased significantly in Val group compared with those in Ram group and Aml group.TRPC6 mRNA and protein expressions decreased in drug intervention groups;however,no significant changes in TRPC6 mRNA and protein expressions were observed.CONCLUSION: Expressions of TRPC3 and TRPC6 were found in SHR and WKY.TRPC3 and TRPC6 may co-regulate the pathphysiological processes of myocardial hypertrophy.Valsartan may inhibit left ventricular hypertrophy,probably by downregulating mRNA and protein expression of TRPC3.