Abstract
The timing of RNA and DNA synthesis in the pupal epidermis of Tenebrio molitor has been investigated both autoradiographically, using tritiated precursors, and by a study of the effects of the antibiotics actinomycin D and mitomycin C on cuticle production. Although three regions of the epidermis, the limbs, the head and thorax, and the abdomen, show different temporal patterns of RNA synthesis, DNA synthesis in all regions appears to be synchronized. These results and the pupal-adult intermediates produced by the injection of both actinomycin D and ouabain are compared with reported effects of juvenile hormone and its analogues on Tenebrio molitor metamorphosis.
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