Effects of anode materials on nitrate reduction and microbial community in a three-dimensional electrode biofilm reactor with sulfate

Abstract

Graphite rod corrosion and peeling are serious problems in three-dimensional electrode biofilm reactors (3D-BERs). In this study, titanium rods, titanium suboxide-coated titanium rods and graphite rods were used as anodes to investigate the effect of anodic materials on the electrochemical and bioelectrochemical reduction of nitrate and sulfate. The results showed that the reactor with the titanium suboxide-coated titanium rod anode (3D-ER-T) exhibited a stable NO3−-N removal efficiency (46%–95%) with a current range of 160–320 mA in the electrochemical reduction process. In the bioelectrochemical reduction, the removal efficiencies of NO3−-N and SO42− and nitrogen selectivity in the 3D-BER with titanium suboxide-coated titanium rod anode (3D-BER-T) were higher than those in the 3D-BER with titanium suboxide-coated graphite rod anode (3D-BER-G). The removal efficiencies of NO3−-N and SO42− and nitrogen selectivity were 92%, 43% and 86%, respectively, in 3D-BER-T under 320 mA and HRT 12 h. Anode materials affected the microbial community. Hydrogenophaga and Dethiobacter were the dominant bacteria in 3D-BER-T, while OPB41 and Sulfurospirillum were dominant in 3D-BER-G. Nitrate and sulfate were effectively removed in 3D-BER-T by the synergistic work of electrochemical reduction, bioelectrochemical reduction and indirect electrochemical reduction. The resupply/reserve mode of the electron donor promoted the load of shock resistance of 3D-BER-T via the sulfur cycle. Titanium suboxide coating could significantly enhance the anti-corrosion ability of matrix anodes.