Abstract
Background and Objectives:The octapeptide hormone of the renin-angiotensin system, angiotensin Ⅱ, regulates a wide variety of physiological responses including salt and water balance, blood pressure, and vascular tone. Contradictory results have been reported regarding the effects of angiotensin Ⅱ on vascular smooth muscle cell (VSMC proliferation. The aim of the present study was to investigate the direct effect of angio- tensin Ⅱ on the growth of VSMC. Materials and Method:Rat aortic smooth muscle cells were obtained by the combined collagenase and elastase methods. Cells between the 4th and 8th passages were used for the experiments. Cultures were treated daily for 3 days with either angiotensin Ⅱ alone or angiotensin Ⅱ with equimolar concentrations of saralasin. Incorporated radioactivity of ( 3 H)thymidine and ( 14 C)phenylalanine was measured by liquid scintillation spectrometry. Results:Angiotensin Ⅱ increased ( 14 C)phenyalanine incor- poration about 20-30%, and saralasin completely blocked the stimulation by angiotensin Ⅱ. However, there was no significant increase in ( 3 H)thymidine incorporation by angiotensin Ⅱ stimulation in this study. Conclusion:These results suggest that angiotensin Ⅱ alone induces cellular hypertrophy but has no detectable mitogenic activity in cultured rat aortic VSMC. ( ( ( (Korean Circulation J 1999;29( ( ( (2 :209-215
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