Effects of angiotensin II, K+, adrenocorticotropin, serotonin, adenosine 3',5'-monophosphate, guanosine 3',5'-monophosphate, A23187, and EGTA on aldosterone synthesis and phospholipid metabolism in the rat adrenal zona glomerulosa.

Abstract

Rat adrenal glomerulosa cells and capsules were incubated in vitro with angiotensin II (A-II), ACTH, K+, (Bu)2cAMP, (Bu)2cGMP, A23187, and serotonin to compare their effects on steroidogenesis and phospholipid metabolism. In both glomerulosa cells and capsules, A-II, ACTH, K+, (Bu)2cAMP, and serotonin enhanced aldosterone or corticosterone production, but only A-II, K+, and serotonin significantly and consistently increased the incorporation of [32P]Pi into phosphatidic acid (PA) and phosphatidylinositol (PI). A-II, ACTH, K+, (Bu)2cAMP, and serotonin all provoked nearly 2- fold increases in PI mass in adrenal capsules. (Bu)2cGMP had relatively small effects, if any, on aldosterone production or glomerulosa phospholipid metabolism. In adrenal capsules, the effects of K+ on [32P]Pi incorporation into PA and PI were inhibited by EGTA, but the effects of A-II on [32P]Pi incorporation were not influenced by EGTA, despite observed inhibition of steroidogenesis. Cycloheximide also inhibited steroidogenesis, but ...