Effects of androgen receptor gene on adhesion and invasion of human bladder cancer T24 cells

Abstract

Objective To study the effects of androgen receptor (AtR) gene small interfering RNA (siRNA) on adhesion and invasion of human bladder cancer cells.Methods Human bladder cancer T24 cells with AR expression were transfected with AR-siRNA.The expression of AR mRNA and protein was detected by using real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting,respectively.The cell adhesion was evaluated by using methyl thiazol tetrazolium (MTT) assay,and invasion was examined by Transwell chamber.Results T24 cells with AR expression were successfully transfected with AR-siRNA.The Real-time PCR and Western blotting revealed that the expression of AR mRNA and protein was reduced.The adhesive rate in AR-siRNA group was (38.7 ± 4.4) ％,lower than that in blank control group (P ＜ 0.05).The Transwell results showed that the invasion cells were (30.5 ± 6.7)and (59.2 ± 8.1) in AR-siRNA and blank control groups,respectively (P ＜ 0.05).Conclusion AR gene might play an important role in adhesion and invasion of human bladder cancer cells.siRNA targeted AR could effectively inhibit adhesion and invasion of human bladder cancer. Key words: Bladder carcinoma; Androgen receptor; RNA interference; Invasion