Abstract
The aim of this study was to observe the effects of Cyprinus carpio decoction on the expression of aquaporins in rats with adriamycin-induced nephropathy and to explore the therapeutic mechanism on nephrotic edema. Total of 50 Wistar rats were randomly divided into normal group, model group, fosinopril group, Cyprinus carpio decoction treated with high dose group and low dose group consisting of 10 rats respectively. Nephropathy models were established by injecting adriamycin through tail vein and treated with Cyprinus carpio decoction. Urinary protein excretions in 12 h, serum albumin, total serum protein, serum sodium and potassium were measured by biochemical assay. The pathological changes and the expression of AQP1, AQP2, AQP3 inrat kidneys were respectively detected by HE stain and immunohistochemiscal assay. The results indicated: 1) The urinary protein excretion in 12 h (proteinuria) increased significantly along the time longed modeling, while no significant increasing in Cyprinus carpio decoction treated group (F = 5.23 - 41.89, P Cyprinus carpio decoction treated group were higher than that in model group (F = 13.12 - 15.48, P Cyprinus carpio decoction treated group were higher than that in model group (F = 3.42 - 3.96, P Cyprinus carpio decoction with interventing groups reduced proliferation, less inflammatory cell infiltration; 4) In model group, the expressions of AQP1-3 in the renal tubule and collecting duct cells increased significantly than those in normal group, and those in Cyprinus carpio decoction treated group decreased than those in model group (F = 3.97 - 6.19, P Cyprinus carpio decoction could reduced the urinary protein excretion and alleviate pathological lesion and edema with adriamycin-induced nephropathy by decreasing the expressions of AQPs in kidneys.
Highlights
Nephrotic syndrome (NS) is a clinical syndrome due to a series of pathophysiological changes of high glomerular filtration permeability and a large number of plasma proteins from the urine loss with edema, proteinuria, and low protein hyperlipidemia for the clinical features
Total of 50 Wistar rats were randomly divided into normal group, model group, fosinopril group, Cyprinus carpio decoction treated with high dose group and low dose group consisting of 10 rats respectively
AQP1 was mainly expressed in the cell membrane surface of the renal proximal tubule and medullary descending thin section to reabsorb water from the original urine; AQP2 played an important role in water balance of the kidney, which was mainly distributed in the kidney distal tubule and the lumen side of the main collecting duct cells, controlled by arginine vasopressin (AVP) regulation [2]
Summary
Nephrotic syndrome (NS) is a clinical syndrome due to a series of pathophysiological changes of high glomerular filtration permeability and a large number of plasma proteins from the urine loss with edema, proteinuria, and low protein hyperlipidemia for the clinical features. Water channel proteins (Aquaporin, AQP) are a family of transmembrane proteins through which the water molecules can pass . There are eight kinds of water channel proteins in kidneys, which are important to renal re-absorption of water liquid and dilute urine concentration function. Abnormal expression of AQPs is one of the mechanisms watersodium retention [1]. AQP1 was mainly expressed in the cell membrane surface of the renal proximal tubule and medullary descending thin section to reabsorb water from the original urine; AQP2 played an important role in water balance of the kidney, which was mainly distributed in the kidney distal tubule and the lumen side of the main collecting duct cells, controlled by arginine vasopressin (AVP) regulation [2]. AQP2 was an important protein to regulate
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