Effects of ammonium on the activity and community of methanotrophs in landfill biocover soils

Abstract

The influence of NH4+ on microbial CH4 oxidation is still poorly understood in landfill cover soils. In this study, effects of NH4+ addition on the activity and community structure of methanotrophs were investigated in waste biocover soil (WBS) treated by a series of NH4+-N contents (0, 100, 300, 600 and 1200mgkg−1). The results showed that the addition of NH4+-N ranging from 100 to 300mgkg−1 could stimulate CH4 oxidation in the WBS samples at the first stage of activity, while the addition of an NH4+-N content of 600mgkg−1 had an inhibitory effect on CH4 oxidation in the first 4 days. The decrease of CH4 oxidation rate observed in the last stage of activity could be caused by nitrogen limitation and/or exopolymeric substance accumulation. Type I methanotrophs Methylocaldum and Methylobacter, and type II methanotrophs (Methylocystis and Methylosinus) were abundant in the WBS samples. Of these, Methylocaldum was the main methanotroph in the original WBS. With incubation, a higher abundance of Methylobacter was observed in the treatments with NH4+-N contents greater than 300mgkg−1, which suggested that NH4+-N addition might lead to the dominance of Methylobacter in the WBS samples. Compared to type I methanotrophs, the abundance of type II methanotrophs Methylocystis and/or Methylosinus was lower in the original WBS sample. An increase in the abundance of Methylocystis and/or Methylosinus occurred in the last stage of activity, and was likely due to a nitrogen limitation condition. Redundancy analysis showed that NH4+-N and the C/N ratio had a significant influence on the methanotrophic community in the WBS sample.