Effects of ammonium ion derived from bovine endothelial cells upon low density lipoprotein degradation in cultured vascular smooth muscle cells

Abstract

Low density lipoprotein (LDL) metabolism in bovine arterial smooth muscle cells (SMC) was increased upon exposure to endothelial cell conditioned medium. The mass of LDL degraded in the SMC lysosomal system was increased, and kinetic analysis demonstrated that the rate constant for LDL degradation arising from receptor-mediated endocytis was unchanged. The effects on LDL metabolism were accompanied by stimulation of DNA synthesis in the SMC. These results are in contrast to reports concerning a porcine endothelial cell system where LDL degradation was inhibited by endothelial-derived NH 4 +. We show that bovine endothelial cells produce insufficient NH 4 + to inhibit LDL degradation and conclude that endothelial cell-derived NH 4 + is unlikely to be a factor affecting LDL metabolism in the bovine vascular cell culture system.